Vol. 2, Issue 2, Part A (2025)

The present research aimed to evaluate the stability and shelf-life of Kwatha preparations a classical Ayurvedic decoction under varying storage conditions, viewed through the lens of Bhaishajya Kalpana. Three experimental storage environments were compared: ambient temperature with light exposure, ambient temperature with light protection, and refrigeration with light protection. Standardized Kwatha preparations were analyzed over a 90-day period for physicochemical parameters (pH, specific gravity, total phenolic content, and total flavonoid content) and microbial stability (total aerobic microbial count). The results revealed significant deterioration of phytochemical constituents and increased microbial load in samples stored under ambient and light-exposed conditions, while refrigerated and light-protected samples retained more than 80% of their initial phenolic and flavonoid content and remained microbiologically stable throughout the study period. Statistical analysis (one-way ANOVA) confirmed that storage condition had a significant impact (p<0.05) on all measured parameters, emphasizing the necessity of temperature and light control for maintaining Kwatha quality. The findings not only substantiate traditional Ayurvedic recommendations for immediate use of decoctions but also offer a modern evidence-based framework to extend their shelf-life through optimized storage and packaging. Practically, the study recommends cold-chain storage, use of amber glass containers, airtight sealing, and implementation of WHO-GMP-compliant manufacturing protocols to ensure product safety, potency, and regulatory compliance. Furthermore, integration of validated stability testing protocols and microbiological monitoring into routine quality control can support the rational standardization of Kwatha formulations. By blending classical Ayurvedic principles with contemporary pharmaceutical validation, this study provides a scientific foundation for improving the safety, efficacy, and global acceptance of traditional Kwatha preparations.